Figure 3 . GMP-compliant reagents improve T cell phenotype and function . Samples taken at day 30 harvest from G-Rex10 flasks (stimulation round 3 + 9 days) analysed as before. (A ) Direct comparison of representative line LAD1 cultured using GMP-compliant reagents and standard culture reagents demonstrated both T cell cultures had comparable expression of most markers, but reduced CD57 expression in GMP-compliant process. (B ) Analysis of mean surface marker expression showed a significantly higher percentage of CD62L+/CD45RO+ Central Memory T cells in GMP-compliant culture (n=4) compared to standard (n=4, p=0.002). Intracellular staining showed no significant difference in (C ) cytokine expression between the two groups, or (D ) relative frequency of differentiated T cell subpopulations by cytokine expression. Data are represented as means ± SEM.