Buffy Coat PBMC Isolation
Donor PBMC were isolated from healthy donor buffy coats by ficoll-paque
(GE Healthcare) density centrifugation (450g for 40 minutes) to set up
flow cytometry panels (surface phenotype and intracellular cytokine
assay). Donor PBMC from n=6 buffy coats were also characterised for
cytokine expression in relation to T cell memory markers (see
intracellular cytokine assay).